Etude d'un procédé d'encapsulation de microARN en milieu supercritique (thèse 2017-2020)
Activités
Encapsulation / microARN / CO2 supercritique / SAS
Publications scientifiques au M2P2
2021
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, Wiley-VCH Verlag, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03334357⟩ Plus de détails...
A batch process for producing lutein-loaded liposomes using supercritical CO2 is studied. The effects of the variation of pressure (10 and 15 MPa), temperature (308, 313, and 318 K), and lutein to lipid ratio (0.5 and 1 wt%) on the liposome average size and size distribution are investigated, as well as on the encapsulation efficiency (EE) of lutein. This process is worked in a repeatable manner and is allowed the production of nanoliposomes with mean diameters (MDs) ranging from 65 ± 33 to 77 ± 40 nm, obtaining lutein EEs ranging from 82.1 ± 3.7% to 91.9 ± 2.9%. Temperature, pressure, and lutein to lipid ratio seem to have no impact on size, size distribution, and EE on formed liposomes. The use of low temperatures and low pressures allows the obtainment of liposomes with diameters less than 100 nm and limits the process energy cost. Moreover, the supercritical CO2-assisted batch process effectively encapsulates lutein into liposome, an antioxidant molecule used for the prevention of retinal damage. Liposomes formed by this supercritical process have the desired characteristics for human target delivery. Practical applications: This work on the optimization of a process for developing liposomes in a supercritical environment has applications in medicine. Indeed, the liposomes formed with this process are nanoliposomes with a size of less than 80 nm. In addition, excellent lutein EEs (hydrophobic molecules) show that the liposomes formed constitute excellent coating matrices for the protection of active ingredients. These reasons make these liposome matrices applicable in nanomedicine (injection of sensitive drugs requiring protection before injection). The elaboration process also makes it possible to form liposomes with desired properties by changing pressure, temperature, or lecithin concentration. Therefore, this work focuses on the properties of liposomes as a function of the operating conditions.
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, Wiley-VCH Verlag, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03334357⟩
Journal: European Journal of Lipid Science and Technology
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, Elsevier, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩ Plus de détails...
Supercritical CO2 extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100-300] bar, T: [313-333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO2 at 333 K and both 300-400 bar. Extraction curves were modelled using the Sovova's mathematical model.
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, Elsevier, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, Elsevier, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-03232116⟩ Plus de détails...
Supercritical CO2 extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100-300] bar, T: [313-333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO2 at 333 K and both 300-400 bar. Extraction curves were modelled using the Sovova's mathematical model.
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, Elsevier, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-03232116⟩
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, Elsevier, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩ Plus de détails...
Solid residues from thermal treatments of sewage sludge (SS) represent a valuable source of phosphorus (P) for the fertilizer production. This study aims at evaluating the P recovery potential from solid residues obtained from wet air oxidation of SS under subcritical water conditions (WAO residues). A series of P extraction experiments was performed by acidic and alkaline leaching at different liquid to solid ratios. Hot chemical extractions and P fractionations were also carried out to characterize the chemical composition of the WAO residues. The main objectives of this work were to determine the best operating conditions for P extraction, and to describe and understand the kinetics and the main mechanisms leading to P release. The results obtained in this study indicate that 1 M citric acid and 1 M HCl at the liquid to solid ratio of 10 L/kg can extract 61% and 65% of the total P content after 2 h of contact time at room temperature, thus giving P extraction capacities of 81 and 86 g P/kg WAO residues, respectively. The analysis of kinetic data indicates that P extraction with 1 M HCl is faster, but 1 M citric acid can give higher P extraction efficiencies at the equilibrium. The molar ratios of Ca to P of the leachates suggest that P extraction from WAO residues was primarily due to the dissolution of a mixture of various Ca-P complexes. (C) 2019 Elsevier Ltd. All rights reserved.
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, Elsevier, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩
